It will restore the session just where you saved it. You can load the saved file using the menu item Plugins › Tracking › Load a TrackMate file. Since you can save at any time, the resulting file might miss tracks, spots, etc. The Save button creates a XML file that contains all of the data you generated at the moment you click it. For instance, if you go back on the segmentation panel, segmentation will not be re-executed. The Previous button steps back in the process, without executing actions. For instance, if you do not select a valid image in the first panel, it will be disabled. It might be disabled depending on the current panel content. The Next button allows to step through the tracking process. The user interface is a single frame - that can be resized - divided in a main panel, that displays context-dependent dialogs, and a permanent bottom panel containing the four main buttons depicted on the right. The TrackMate GUI appears next to the image, displaying the starting dialog panel.īut first, just a few words about its look. With this image selected, launch TrackMate from the menu Plugins › Tracking › TrackMate or from the Command launcher. You can find it in File › Open Samples › Tracks for TrackMate (807K), at the bottom of the list.Įrror creating thumbnail: Unable to save thumbnail to destination
The test image we will use for this tutorial has now a link in Fiji. In these views, TrackMate resembles a bit to the Spot Segmentation Wizard of Imaris™. So there is a step to filter them out before tracking. Therefore each step is handled in the user interface by a specific panel, and you will go back in forth through them.Īlso, TrackMate has a fishing net with small holes: it will find as much spots as it can, even the ones you are not interested. It follows the classical scheme, where the segmentation step and the particle-linking steps are separated. The TrackMate plugin provides a way to semi-automatically segment spots or roughly spherical objects from a 2D or 3D image, and track them over time. It explains how it works by walking you through a simple case, using an easy image. This tutorial is the starting point for TrackMate users.
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